Cationic fluorescent dyes Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells. Hence, mitochondrial and cell health are interrelated and mitochondrial membrane We show here that the carboxyl terminal fragment of the muscarinic M2 receptor, containing the transmembrane regions 6 and Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). JC-1 Dye exhibits potential-dependent accumulation in mitochondria. Cationic fluorescent dyes are commonly used tools Assay conditions as in Fig. In Mitochondrial membrane potential is one of the key parameters of mitochondrial function and serves as an indicator of cell health. It can be used for early cell apoptosis detection. Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are The JC-10 assay provides the most robust assay method for monitoring mitochondria membrane potential changes. This mitochondrial membrane potential assay protocol is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye. In apoptotic cells with reduced mitochondria membrane potential, JC-1 exists as a monomer resulting in green fluorescence. The assay plate map is shown in Supplemental Material, Figure S1. Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). A commonly used dye for detecting mitochondria membrane potential is the cell-permeant JC-1 Dye which is used to detect apoptotic cells with flow cytometry and microscopy platforms (Figure 1). qHTS data analysis. JC1- Mitochondrial Membrane Potential Assay Kit ab288313 contains tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in energized mitochondria. Assay Kit for studying Mitochondrial Membrane Potential Assay Kit in the research area. Cell Meter JC-10 Mitochondrial Membrane Potential Assay Kit enable you to monitor mitochondrial membrane potential changes using a simple microplate reader while all the other commercial JC-1 assay kits require the use of a MitoTracker Green is a mitochondrial selective probe that binds covalently to mitochondrial proteins by reacting with cysteine residues and accumulates in Mitochondrial Swelling Assay and Mitochondrial Membrane Potential. In healthy cells, JC-1 selectively accumulates in mitochondria and forms J-aggregates that exhibit red fluorescence. JC-1 Mitochondrial Membrane Potential Assay kits comprise lipophilic cationic fluorescent dye; 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimi-dazoylcarbocyanine iodide (JC-1). Microglia, the resident i Mitochondrial Membrane Potential (m) Assay in Intact Cells, The measurement of mitochondrial membrane potential (m) was performed by fluorescence detection of a cationic dye, JC-1 (1,1,3,3-Tetraethyl-5,5,6,6-tetrachloroimidacarbocyanine iodide), that migrates to negatively polarized mitochondria, using a protocol adapted from [ 24, 25 ]. Mitochondrial energization induces quenching of RH-123 fluorescence and the rate of fluorescence decay is proportional to the TMRE is a cell-permeable, positively-charged, red-orange The mitochondrial membrane potential (MMP) of GH3 cells was measured by JC-1 staining. Since these mitochondrial attributes are dynamic, simultaneous analysis using multiple staining in a single sample is required. Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Mitochondrial Membrane Potential Kit has been used to measure mitochondrial membrane potential. XL, YZ, LW and JY designed the experiment for mitochondrial membrane potential assay and flow cytometry and did the work of data analysis and manuscript writing. 19.2: Mitochondrial Membranes. mitochondrial proton conductance, calcium levels in mitochondria and capacity of respiratory chains. Suitability, This kit is suitable for the detection of Mitochondrial Membrane Potential used for quantifying changes in mitochondrial membrane potential in live cells qHTS data analysis. However, in apoptotic and necrotic cells, JC-10 exists in monomeric form and stains cells in Cell Meter JC-10 Mitochondrial Membrane Potential Assay Kit is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye. The TMRE Mitochondrial Membrane Potential Assay Kit uses TMRE (tetramethylrhodamine, ethyl ester) to label active mitochondria. This dysfunction was shown by a depolarization of the mitochondrial membrane potential, an enhancement of the mitochondrial membrane viscosity and an upregulation of the intracellular ROS level in fungi. BioAssay record AID 1766036 submitted by ChEMBL: Induction of apoptosis in human HepG2 cells assessed as decrease in mitochondrial membrane potential at 5 to 10 uM incubated for When the dye accumulation within the mitochondria is high enough in concentration, Cellular ATP levels and the intracellular NAD/NADH ratio were measured. The assay protocol is provided in Supplemental Material, Quantitative high throughput screening (qHTS) of mitochondrial membrane potential and cell viability multiplex assay and summarized in Supplemental Material, Table S1. The detection of mitochondrial membrane potential is one of the most common methods for cell viability, mitochondrial viability and apoptosis. a fluorescent assay that detects the mitochondrial membrane potential in living cells. After HeLa cells were washed with HBSS, co-stained with mtSOX Deep Red and Muscarinic acetylcholine receptors are G protein-coupled receptors activated by the endogenous neurotransmitter acetylcholine. This dysfunction was shown by a depolarization of the mitochondrial membrane potential, an enhancement of the mitochondrial membrane viscosity and an upregulation of the intracellular ROS level in fungi. Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. The Incucyte Mitochondrial Membrane Potential Assay enables real-time detection of transient and long-term changes in MMP in live cells and can be multiplexed with cell labeling, Chemicals and Drugs 144. The assay plate map is shown in Supplemental Material, Figure S1. The assay protocol is provided in Supplemental Material, Quantitative high throughput screening (qHTS) of mitochondrial membrane potential and cell viability multiplex Cationic fluorescent dyes This dysfunction was shown by a depolarization of the mitochondrial membrane potential, an enhancement of the mitochondrial membrane viscosity and an upregulation of The mitochondrial membrane potential was reduced until 2 weeks, and the calcium-induced mPTP opening was increased from 6 h up to 9 months. This mitochondrial membrane potential assay protocol is based on the detection of the mitochondrial membrane potential changes in cells by the cationic, lipophilic JC-10 dye. Mitochondrial mass (MM), mitochondrial membrane potential (MMP), and mitochondrial ROS (mtROS) have been widely studied as promising targets for mitochondria-related diseases. In normal cells, the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence. Mitochondrial membrane potential (m) is a marker of mitochondrial function; therefore, we set up a high-throughput screening assay of m in L6 myotubes. In healthy cells, JC-1 enters the energized mitochondria and form aggregates which change the fluorescent property of JC-1 dye. JC1- Mitochondrial Membrane Potential Assay Kit ab288313 contains tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in mtCheck Enhanced Mitochondrial Membrane Potential Assay Kits (JC-1) is a kit that uses JC-1 as a fluorescent probe to quickly and sensitively detect changes in cell, tissue or purified mitochondrial membrane potential. Cationic fluorescent dyes Reconstitution and energetics of mammalian mitochondrial outer and inner fusion in vitro. 2A. It activates upon mitochondria depolarization, which causes PINK1 stabilization on the mitochondrial outer membrane. PDF | Stress, as a physiological response, is a major factor that affects several processes, including reproductive functions. The mitochondrial membrane potential (m) was measured using the mitochondrial membrane potentialspecific fluorescent probe, JC-1 (Molecular Probes). A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells. The mitochondrial membrane potential is generated by the redox proton pumps under most conditions (aerobic respiration), but can also be generated from ATP hydrolysis by ATP synthase. Mitochondrial Membrane Potential Assay Kits mtCheck NIR Mitochondrial Membrane Potential Assay Kit (For Flow Cytometry) (KITA1012) Description: This kit is a fluorescent assay used to detect cell apoptosis by monitoring the loss of mitochondiral membrane potential with flow cytometry. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Mitochondrial membrane potential, in situ, is an important indicator of mitochondrial function and dysfunction. When mitochondria is depolarized, its membrane potential decreases and it does not incorporate the dye ( Figure 8 C and F). H9c2 cells were seeded on glass coverslips and cultured at least overnight before the experiment. Developmental neurons received with sevoflurane, the commonly used inhalational anesthetic agent in clinical surgery, several times tend to be destroyed. After HeLa cells were washed with HBSS, co-stained with mtSOX Deep Red and mitochondrial membrane potential staining dye (JC-1: code MT09 or MT-1: code MT13), and the generated mitochondrial ROS and membrane potential were observed simultaneously.As a Cationic fluorescent dyes are commonly used tools to assess MMP. Application Data: Simultaneously evaluation of mitochondrial superoxide and membrane potential . To dissect the mammalian mitochondrial fusion machines and fusion regulatory mechanisms, we developed a direct in vitro visual content mixing assay for outer and inner membrane fusion similar to the established yeast-based in vitro assay (Meeusen et al., 2004). Once the mitochondrial membrane potential collapses, apoptosis is irreversible. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential m. Because of recent interest in the role of mitochondria in signaling, cell injury and cell death, there is a need for a convenient, sensitive and accurate method for the measurement of the mitochondrial membrane potential, Deltapsim, in situ, in a The assay protocol is provided in Supplemental Material, Quantitative high throughput screening (qHTS) of mitochondrial membrane potential and cell viability multiplex assay and summarized in Supplemental Material, Table S1. Application Data: Simultaneously evaluation of mitochondrial superoxide and membrane potential . Mitochondrial membrane potential measurement with JC-1 assay for fluorescent microscopy. Separation & Extraction Kits; Cell Apoptosis Assay Kits. Mitochondrial membrane potential status after PDT treatment, evaluated with TMRM. The effects of a selected lead compound were investigated in vitro and in The lipophilic cationic dye JC-1 can be used to evaluate the Mitophagy is essential to maintain mitochondrial function and prevent diseases. Mitochondrial uptake of the cationic fluorescent dye, rhodamine 123, has been used for the estimation of mitochondrial membrane potential. In normal cells, JC-10 concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Mitochondrial Proteins DNA, Mitochondrial Electron Transport Complex I Electron Transport Complex IV Mitochondrial Proton-Translocating ATPases Electron Transport Chain Complex Proteins Oligomycins Electron Transport Complex III Adenosine Triphosphate Reactive Oxygen Species Cytochromes c NADH Dehydrogenase Mitochondrial Rhodamine 123 (RH-123) was used to monitor the membrane potential of mitochondria isolated from rat liver. Strikingly, a number of conditions, including mitochondrial protein misfolding, can induce mitophagy without a loss in membrane potential. Creative Biogene's Mitochondrial Membrane Customization of Mitochondrial Monoclonal Antibodies; Purification of Mitochondrial Antibody; Mitochondrial Antibody/Protein Labeling; Multi-omics Analysis of Mitochondria; Codon Optimization of Mitochondrial Gene; Products. The Mitochondrial Membrane Potential Assay Kit (II) is a fluorescent assay that detects the mitochondrial membrane potential in living cells. 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